Application of Endoxylanases of Bacillus halodurans for Producing Xylooligosaccharides from Empty Fruit Bunch

Endo-1,4-β-xylanase catalyzes the random hydrolysis of β-1,4-D-xylosidic bonds in xylan, resulting formation oligomers xylose. This study aims to demonstrate promise endoxylanases from alkaliphilic Bacillus halodurans for production xylooligosaccharides (XOS) oil palm empty fruit bunch (EFB) at high pH. Two enzyme preparations were employed: recombinant endoxylanase Xyn45 (GH10 xylanase) and nonrecombinant endoxylanases, a mixture two extracellular endo-1,4-β-xylanases Xyn23 (GH11 produced by B. halodurans. EFB was first treated with an alkaline solution. Then, dissolved xylan-containing fraction retained, prepared added react pH 8 convert xylan into XOS. Compared use only Xyn45, combined resulted higher yield XOS, suggesting synergistic effect endoxylanases. The XOS obtained as 46.77% ± 1.64% (w/w), xylobiose-to-xylotriose ratio being 6:5. However, when activity dose low, product contained more xylotriose than xylobiose. Four probiotic lactobacilli bifidobacteria grew well on medium containing EFB. presence increased cell mass reduced pH, that promoted growth probiotics.